C3787

Chloramphenicol succinate sodium salt

≥80% (HPLC)

• Sinónimos: Chloramphenicol α-succinate
• Fórmula lineal: C₁₅H₁₅Cl₂N₂O₈Na
• Número de CAS: 982-57-0
• Peso molecular: 445.18
• Número CE: 213-568-1
• Número MDL: MFCD00079025
• Código UNSPSC: 41116107
• ID de la sustancia en PubChem: 24892643
• NACRES: NA.85

Propiedades

• Análisis: ≥80% (HPLC)
• formulario: solid
• solubilidad: H₂O: 50 mg/mL
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria
• Modo de acción: protein synthesis | interferes
• cadena SMILES: [Na].O[C@@H]([C@@H](COC(=O)CCC(O)=O)NC(=O)C(Cl)Cl)c1ccc(cc1)N(=O)=O
• InChI: 1S/C15H16Cl2N2O8.Na.H/c16-14(17)15(24)18-10(7-27-12(22)6-5-11(20)21)13(23)8-1-3-9(4-2-8)19(25)26;;/h1-4,10,13-14,23H,5-7H2,(H,18,24)(H,20,21);;/t10-,13-;;/m1../s1
• Clave InChI: RJOAHMNSYANTPN-OWVUFADGSA-N

Descripción

Descripción general

Chemical structure: phenicole

Aplicación

Chloramphenicol is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Acciones bioquímicas o fisiológicas

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Precaución

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

Nota de preparación

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

Otras notas

Keep container tightly closed in a dry and well-ventilated place.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302,H351
• Consejos de prudencia: P202 - P264 - P280 - P301 + P312 - P308 + P313 - P405
• Clasificaciones de peligro: Acute Tox. 4 Oral - Carc. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges