C3535
Complement C8 from human serum
suitable for radioiodination, RIA, ≥85% (SDS-PAGE), ≥125,000 C8H50 units/mg protein (using C8 deficient serum)
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 0.1 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 333,00 € |
Acerca de este artículo
Form:
solution
Assay:
≥85% (SDS-PAGE)
Biological source:
human
333,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarlebiological source
human
Quality Level
assay
≥85% (SDS-PAGE)
form
solution
specific activity
≥125,000 C8H50 units/mg protein (using C8 deficient serum)
concentration
1 mg/mL in PBS, pH 7.2
technique(s)
immunoelectrophoresis: suitable
suitability
suitable for radioiodination, RIA
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... C8A(731)
Application
Complement C8 is one of the end terminals of the complement system contained in the membrane attack complex (MAC). In the (MAC), C8 initiates and coordinates MAC pore formation. It has been used in research to investigate the mechanisms of how C8-C9 and C9-C9 interactions facilitate folding of the membrane attack complex perforin (MACPF) domain to form the circular pore.
Biochem/physiol Actions
C8 is one of the components of the terminal complement complex and is required for a functional complement system. It is composed of disulfide-linked C8α and C8γ and a noncovalently associated C8β chain. During formation of the membrane-attack complex, a domain on C8α induces C9 to polymerize and form a pore-like structure.
Analysis Note
Functionally pure by a sensitive hemolytic assay using depleted sera.
Other Notes
View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Este artículo | |||
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human serum |
| technique(s) immunoelectrophoresis: suitable | technique(s) - | technique(s) activity assay: suitable | technique(s) activity assay: suitable |
| assay ≥85% (SDS-PAGE) | assay ≥85% (SDS-GE) | assay ≥85% (SDS-GE) | assay ≥90% (SDS-PAGE) |
| concentration 1 mg/mL in PBS, pH 7.2 | concentration - | concentration - | concentration 1 mg/mL in PBS, pH 7.2 |
| form solution | form solution | form solution | form solution |
| suitability suitable for radioiodination, RIA | suitability - | suitability - | suitability - |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Daniel J Slade et al.
Biochemistry, 45(16), 5290-5296 (2006-04-19)
Human C8 is one of five components of the membrane attack complex of complement (MAC). It contains three subunits (C8alpha, C8beta, C8gamma) arranged as a disulfide-linked C8alpha-gamma dimer that is noncovalently associated with C8beta. C8alpha, C8beta, and complement components C6
Doryen Bubeck et al.
Journal of molecular biology, 405(2), 325-330 (2010-11-16)
Complement component C8 plays a pivotal role in the formation of the membrane attack complex (MAC), an important antibacterial immune effector. C8 initiates membrane penetration and coordinates MAC pore formation. High-resolution structures of C8 subunits have provided some insight into
Leslie L Lovelace et al.
The Journal of biological chemistry, 286(20), 17585-17592 (2011-04-02)
C8 is one of five complement proteins that assemble on bacterial membranes to form the lethal pore-like "membrane attack complex" (MAC) of complement. The MAC consists of one C5b, C6, C7, and C8 and 12-18 molecules of C9. C8 is
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| C3535-.1MG | 04061833420171 |