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C-28030

Sigma-Aldrich

Mononuclear Cell Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

formulario

liquid

envase

pkg of 500 ml

técnicas

cell culture | mammalian: suitable

Condiciones de envío

wet ice

Descripción general

Mononuclear Cell Medium (Ready-to-use)

Aplicación

Mononuclear Cell Medium is a very rich short-term maintenance medium, which provides utmost cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood and other sources, e.g. bone marrow. It is suitable to maintain Mononuclear Cells for up to 48 hours before proceeding with your experiments.Recommended for:Human Mononuclear Cells from Cord Blood (hMNC-CB)Human Mononuclear Cells from Peripheral Blood (hMNC-PB)

Calidad

All lots of PromoCell Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of PromoCell Mononuclear Cell Medium is checked for unaltered shortterm maintenance of highly viable mononuclear cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparación

The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium.

Almacenamiento y estabilidad

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix is delivered thawed and can be frozen after arrival without losing any activity

Rutina de subcultivo

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Código de clase de almacenamiento

10 - Combustible liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Protocolos

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

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