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Merck
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C-27020

Sigma-Aldrich

Osteoblast Mineralization Medium

Ready-to-use kit including Basal Medium and SupplementMix, 100 ml

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

formulario

liquid

técnicas

cell culture | mammalian: suitable

Condiciones de envío

wet ice

Descripción general

Osteoblast Mineralization Medium (Ready-to-use)

Aplicación

Osteoblast Mineralization Medium has been developed for the efficient induction of strong mineralization in human osteoblast cultures. Extensive extracellular calcium-deposits can be observed after 21 days by following an easy one-step protocol. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)

Calidad

All lots of PromoCell Osteoblast Mineralization Medium are tested for their capacity to induce mineralization in primary human osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparación

Contains all the growth factors and supplements necessary for mineralization of human osteoblasts.

Almacenamiento y estabilidad

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutina de subcultivo

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Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Contenido relacionado

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

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