Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture. The medium is optimized for primary human cells but can also be used for primary porcine cardiac myocytes.Recommended for:Human Cardiac Myocytes (HCM)
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 0.5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 2 ng / ml Insulin (recombinant human): 5 µg / ml
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis Note
All lots of PromoCell Myocyte Growth Medium are subjected to comprehensive quality control tests using primary human cardiac myocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested myocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Other Notes
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
