C-12561

Human Bronchial Smooth Muscle Cells (HBSMC)

500,000 cryopreserved cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human bronchi
• Morphology: (smooth muscle)
• Relevant disease(s): asthma

Propiedades

• biological source: human bronchi
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (smooth muscle)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): asthma
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HBSMC are now also available from HLA-typed and COPD/Asthma donors.

Application

Primary Human Bronchial Smooth Muscle Cells (HBSMC) are isolated from the bronchi from single donors and are provided in a cryopreserved format. HBSMC stain positive for smooth muscle α-actin. They connect the endings of the bronchial cartilage and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the bronchi walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HBSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Biochem/physiol Actions

Bronchi

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable