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C-12385

Sigma-Aldrich

Human Uterine Fibroblasts (HUF)

500,000 cryopreserved cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

origen biológico

human uterus (myometrium)

envase

pkg of 500,000 cells

morfología

(fibroblast)

técnicas

cell culture | mammalian: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−196°C

Descripción general

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origen línea celular

Uterus

Aplicación

Primary Human Uterine Fibroblasts (HUF) are isolated from the uterine wall and provided in a cryopreserved format. During pregnancy, the uterine wall undergoes deep but reversible structural and biochemical changes. The uterine wall is the target of hormones and growth factors that act simultaneously and lead to extensive modifications of the extracellular matrix. This and other morphological and biochemical effects can be studied in vitro using HUF.

Calidad

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutina de subcultivo

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Otras notas

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Productos recomendados

Recommended Primary Cell Culture Media:Link

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Artículos

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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