Biotin is soluble directly in water at 0.22 mg/mL, a 1 mg/mL solution is water is not achievable. However, this product is tested for solubility at 50 mg/mL in 2 N NaOH. Concentrated stock solutions prepared in NaOH may then be diluted in buffer, medium, or water to the desired concentration. The solution stability, when stored at ?20 °C, has not been investigated. The end user would have to determine stability. As a general practice, avoid repeated freeze/thaw cycles. Note that some versions of classical medium, such as Ham’s F12 (see product N6658) include D-Biotin. These sterile liquid media are stable for approximately 1 year at 2–8 °C. Similar stability would be expected for sterile Biotin stock solutions.
B4639
Biotina
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Oferta disponible
Sinónimos:
D-Biotina, Bios II, Coenzima R, Vitamina H, Vitaminas B7
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
100 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 52,40 € |
500 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 159,00 € |
1 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 240,00 € |
5 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 1030,00 € |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C10H16N2O3S
Número CAS:
Peso molecular:
244.31
NACRES:
NA.21
PubChem Substance ID:
eCl@ss:
34058011
UNSPSC Code:
12352201
EC Number:
200-399-3
MDL number:
Beilstein/REAXYS Number:
86838
52,40 €
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Servicio técnico
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Permítanos ayudarleproduct line
BioReagent
Quality Segment
assay
≥99%
form
powder
mol wt
Mw 244.31 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
mp
231-233 °C (lit.)
solubility
H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
storage temp.
2-8°C
SMILES string
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
InChI key
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
General description
La biotina es una vitamina del complejo B. Es un compuesto bicíclico con un peso molecular de 244,31 Da. La biotina actúa como un cofactor importante para las carboxilasas de mamífero. Se encuentra dentro de las células en forma libres y unida.
Application
La biotina se ha utilizado:
- como un suplemento de los medios M199 en sistemas de cultivo in vitro
- como un componente del medio exento de suero SATO para el aislamiento de los precursores de los oligodendrocitos corticales (OPC)
- en cultivo con precursores bioquímicos para la síntesis de novo de ácidos grasos
- como un componente en los medios de cultivo agregados
Biochem/physiol Actions
Vitamina esencial que es importante para el metabolismo de aminoácidos y energético, y para la síntesis de ácidos grasos. Constituye un grupo prostético para cuatro familias de carboxilasas de mamífero y facilita la unión y la transferencia del dióxido de carbono.
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Este artículo | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99% | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland | description European Pharmacopoeia (EP) Reference Standard | description ≥99% (HPLC), lyophilized powder |
| technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) LC/MS: suitable, gas chromatography (GC): suitable, HPLC: suitable | technique(s) liquid chromatography (LC): suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable |
| assay ≥99% | assay - | assay - | assay ≥99% (HPLC) |
| form powder | form - | form solid | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| solubility H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.) | solubility - | solubility - | solubility ammonium hydroxide: 50 mg/mL (2 M) |
| mp 231-233 °C (lit.) | mp 231-233 °C (lit.) | mp 231-233 °C (lit.) | mp 231-233 °C (lit.) |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can I dilute it in H2O for 1mg/ml stock concentration, aliquot and store at -20C?
1 respuesta-
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How can I determine the shelf life / expiration / retest date of this product?
1 respuesta-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf¿Le ha resultado útil?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 respuesta-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If I would like to make 1M biotin stock (B4639), what would be the best way?
1 respuesta-
A 1 M solution of biotin would require a concentration of 244 mg/mL. The maximum solubility tested for this compound is 50mg/ml in 2M NaOH or NH4OH, which results in a 204 mM solution. Similar rates have been reported for DMSO. Heat and agitation may improve the solubility. However, a precipitate may form upon cooling. A search of the literature did not reveal a more efficient solvent for Biotin. The preparation of a 1M solution would have to be determined experimentally.
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Hello, How can I reconstruct biotin powder (B4639)? which solvent? and how can I prepare 50mM biotin solution for 1 ml final solution?
1 respuesta-
This product is soluble in 50 mg/ml 2 M NH4OH.
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