B3150

Monoclonal Anti-Protein A−Biotin antibody produced in mouse

clone SPA-27, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Monoclonal Anti-Protein A
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: mouse
• conjugado: biotin conjugate
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: SPA-27, monoclonal
• formulario: buffered aqueous solution
• técnicas: direct ELISA: 1:80,000-1:160,000
• isotipo: IgG1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Especificidad

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Inmunógeno

protein A from Cowan I strain of Staphy­lococcus aureus.

Aplicación

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Acciones bioquímicas o fisiológicas

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Nota de preparación

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.

Almacenamiento y estabilidad

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable