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A9415

Sigma-Aldrich

Actinomycin D

from Streptomyces sp., suitable for cell culture, ≥95%

Sinónimos:

Cosmegen, Meractinomycin, Actinomycin C1, Actinomycin IV, Dactinomycin

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About This Item

Fórmula empírica (notación de Hill):
C62H86N12O16
Número de CAS:
50-76-0
Peso molecular:
1255.42
Beilstein:
605235
Número CE:
200-063-6
Número MDL:
MFCD00005033
Código UNSPSC:
51282001
ID de la sustancia en PubChem:
24891447
NACRES:
NA.76

origen biológico

Streptomyces sp.

Nivel de calidad

300

Ensayo

≥95%

Formulario

powder

técnicas

cell culture | mammalian: suitable

color

red, powder

mp

251-253

solubilidad

ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)

espectro de actividad antibiótica

neoplastics

Modo de acción

DNA synthesis | interferes

temp. de almacenamiento

2-8°C

cadena SMILES

CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O

InChI

1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1

Clave InChI

RJURFGZVJUQBHK-IIXSONLDSA-N

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Categorías relacionadas

Descripción general

Chemical structure: peptide

Aplicación

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells.. Recommended for us in cell culture applications at 1μg/mL.

Acciones bioquímicas o fisiológicas

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Precaución

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

Nota de preparación

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Otras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Pictogramas

Skull and crossbonesHealth hazard
GHS06,GHS08

Palabra de señalización

Danger

Frases de peligro

H300,H350,H360FD

Clasificaciones de peligro

Acute Tox. 2 Oral - Carc. 1B - Repr. 1B

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificados de análisis (COA)

Lot/Batch Number
0000375979
0000375979
0000325066
0000327286
089M4017V

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Angela Zarama et al.
PLoS pathogens, 10(3), e1004000-e1004000 (2014-03-15)
Receptors of the signalling lymphocyte-activation molecules (SLAM) family are involved in the functional regulation of a variety of immune cells upon engagement through homotypic or heterotypic interactions amongst them. Here we show that murine cytomegalovirus (MCMV) dampens the surface expression
Arkady Khoutorsky et al.
Neuron, 78(2), 298-311 (2013-04-30)
Control of protein synthesis is critical for synaptic plasticity and memory formation. However, the molecular mechanisms linking neuronal activity to activation of mRNA translation are not fully understood. Here, we report that the translational repressor poly(A)-binding protein (PABP)-interacting protein 2A
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