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A5670

Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Pig Antibodies, Anti-Pig Antibodies - Anti-Pig IgG (whole molecule)-Peroxidase antibody produced in rabbit, Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit

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Talla/SKUDisponibilidadPrecio
1 mL
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201,00 €

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
22

201,00 €


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biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

pig

technique(s)

direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. IgG is a major class of immunoglobulin. It has five subclasses of IgG- IgG1, IgG3, IgG2a and IgG2b. Affinity isolated antibody is obtained from rabbit anti-pig IgG antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to pig IgG. Anti-Pig IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.

Immunogen

purified pig IgG

Application

Anti-Pig IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. Pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-pig IgG (whole molecule)-peroxidase antibody binds to pig IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo
A5545P0916A5295
description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

whole antiserum

description

affinity isolated antibody, buffered aqueous solution

technique(s)

dot blot: 1:150,000 (chemiluminescent), direct ELISA: 1:40,000

technique(s)

direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

technique(s)

quantitative precipitin assay: 2.8 mg/mL, indirect ELISA: 1:40,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, dot blot: 1:80,000 (chemiluminescent), direct ELISA: 1:20,000

species reactivity

pig

species reactivity

guinea pig

species reactivity

-

species reactivity

bovine

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

whole antiserum

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution


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Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

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WGK 2



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Preguntas

  1. Will Anit-Pig IgG have the same ability to bind to antibodies from all pig breeds? Including minipigs, or potbellied pigs?

    1 respuesta
    1. Unfortunately, the ability of this product to bind to all pig breeds has not been tested.

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