A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Sinónimos:
Acid Alcohol
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About This Item
Productos recomendados
formulario
solution
caducidad
40 mo.
color
colorless
aplicaciones
hematology
histology
temp. de almacenamiento
room temp
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Descripción general
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Aplicación
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
- the prediction of pulmonary metastasis progression in osteosarcoma patients
- the development of multispectral imaging to detect melanin
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Características y beneficios
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
Principio
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
Órganos de actuación
Eyes,Central nervous system
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
71.6 °F
Punto de inflamabilidad (°C)
22 °C
Certificados de análisis (COA)
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