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83200

Pyronin Y

suitable for microscopy

Sinónimos:

Pyronin G, Pyronin J

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Fórmula empírica (notación de Hill):
C17H19ClN2O
Número CAS:
92-32-0
Peso molecular:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57652949
MDL number:
MFCD00011725
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005

mp

250-260 °C (lit.)

solubility

water: 0.01 g/10 mL, red

εmax

≥1650 at 543-553 nm in 50% ethanol, ≥650 at 249-259 nm in 50% ethanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Preguntas

1–5 de 5 Preguntas  

  1. What is the emission wavelength of Pyronin Y, Product 83200?

    1 respuesta

    1. The emission is at 570 nm when dissolved in water.

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  2. Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?

    1 respuesta

    1. No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable.  Product No. P9172 has been used to stain RNA in polyacrylamide gels.  Product No. 213519 was submitted to the Biological Stain Commission for certification.  Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.

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  3. Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?

    1 respuesta

    1. Yes, product HT70116 is available in pre-made form.  The product is approved for in vitro diagnostic (IVD) use.

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  4. What is the solubility of Pyronin Y, Product 83200, in water and ethanol?

    1 respuesta

    1. Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.

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  5. What is the Department of Transportation shipping information for this product?

    1 respuesta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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