The activity and protein concentration of this product varies from lot to lot and are reported in the product Certificate of Analysis. This information may then be used to determine the lot specific volume. As an example, a lot reporting a 99 mg/mL protein concentration would result in a volume of 2.53 mL for the 250 mg package size. This 250 mg package would have a total activity of 2436 units. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/76427#product-documentation
76427
Penicillin Amidase from Escherichia coli
5-10 units/mg protein
Sinónimos:
Penicillin Acylase, Penicillin Amidohydrolase
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250 MG
153,00 €
1 G
451,00 €
153,00 €
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250 MG
153,00 €
1 G
451,00 €
About This Item
153,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Productos recomendados
origen biológico
Escherichia coli
Nivel de calidad
Formulario
suspension
actividad específica
5-10 units/mg protein
mol peso
Mr ~70000
técnicas
activity assay: suitable
aplicaciones
diagnostic assay manufacturing
temp. de almacenamiento
2-8°C
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Descripción general
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.[1]
Aplicación
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.[2] It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.[3] Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.[4]
Acciones bioquímicas o fisiológicas
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.[5] It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.[6]
Definición de unidad
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
Yi-Han Lin et al.
Molecular microbiology, 47(3), 849-860 (2003-01-22)
N-acylhomoserine lactones (AHLs) are used as signal molecules by many quorum-sensing Proteobacteria. Diverse plant and animal pathogens use AHLs to regulate infection and virulence functions. These signals are subject to biological inactivation by AHL-lactonases and AHL-acylases. Previously, little was known
Penicillin Acylase in the Industrial Production of ?-Lactam Antibiotics
Bruggink A, Roos EC, Vroom ED
Organic Process Research & Development, 2(2), 128-133 (1998)
H J Duggleby et al.
Nature, 373(6511), 264-268 (1995-01-19)
Penicillin acylase (penicillin amidohydrolase, EC 3.5.1.11) is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. It is used on an industrial scale for the production of 6-aminopenicillanic acid, the starting material for the synthesis of semi-synthetic penicillins. Its
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Good afternoon, I recently received Penicillin-Amidase aus E. coli 5-10 units/mg protein (250mg). Could you please guide me how much volume does it contain so that we can calculate the precise amount of concenteration available. Thank you in advance.
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