Meridamycin is a non-immunosuppressive, FKBP12-binding natural macrolide with potential therapeutic applications in a variety of medical conditions. To set the stage for structural modification of meridamycin by genetic engineering, we have cloned and completely sequenced approximately 117 kb of DNA
Determination of synthetic polypeptide conformations and molecular geometrical parameters by nonaqueous CE.
L-Lysine cyclodeaminase from Streptomyces pristinaespiralis was heterologously expressed in Escherichia coli, isolated to 90% purity after two purification steps and characterised. The size of the isolated recombinant enzyme was in agreement with the theoretical size calculated from the corresponding gene.
Poly(Nepsilon-trifluoroacetyl-L-lysine) was used as a model solute to investigate the potential of nonaqueous capillary electrophoresis (NACE) for the characterization of synthetic organic polymers. The information obtained by NACE was compared to that derived from size exclusion chromatography (SEC) experiments, and
Journal of chromatography. A, 1159(1-2), 28-41 (2007-02-24)
Nonaqueous background electrolytes broaden the application of capillary electrophoresis displaying altered separation selectivity and interactions between analytes and buffer additives compared to aqueous background electrolytes. In addition, nonaqueous capillary electrophoresis (NACE) appears to be ideally suited for online coupling with
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