51435C

Medio de Eagle modificado de Dulbecco, glucosa elevada

DMEM/High Modified, with 4500 mg/L dextrose, with 110 mg/L sodium pyruvate, without ferric nitrate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Sinónimos: DME, DMEM
• NACRES: NA.75
• UNSPSC Code: 12352207

Propiedades

• Quality Segment: 500
• description: for research or for further manufacturing use
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable, cell culture | stem cell: suitable
• components: L-glutamine: no; glucose: high; phenol red: yes; sodium pyruvate: yes
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

Dulbecco′s Modified Eagle′s Medium has high concentration of amino acids and vitamins.

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s modified eagle′s medium/high modified has been used in the characterisation of nanoparticles in cell culture media.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable