47671

Formamida

BioUltra, for molecular biology, ≥99.5% (T)

• Sinónimos: Amida fórmica, Amidas C₁
• Fórmula lineal: HCONH₂
• Número de CAS: 75-12-7
• Peso molecular: 45.04
• Beilstein: 505995
• Número CE: 200-842-0
• Número MDL: MFCD00007941
• Código UNSPSC: 12352111
• ID de la sustancia en PubChem: 57651075
• NACRES: NA.25

Propiedades

• grado: for molecular biology
• densidad de vapor: 1.55 (vs air)
• presión de vapor: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Línea del producto: BioUltra
• Análisis: ≥99.5% (T)
• formulario: liquid
• temp. de autoignición: 932 °F
• técnicas: FISH: suitable; hybridization: suitable; protein extraction: suitable
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• residuo de ign.: ≤0.1%
• índice de refracción: n20/D 1.447 (lit.); n20/D 1.447
• pH: 7-9 (25 °C, 1 M in H₂O)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• solubilidad: H₂O: 10 M at 20 °C, clear, colorless
• densidad: 1.134 g/mL at 25 °C (lit.)
• trazas de anión: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• trazas de catión: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• cadena SMILES: NC=O
• λ: 10 M in H₂O
• Absorción UV: λ: 260 nm A_max: ≤0.4; λ: 280 nm A_max: ≤0.1
• aplicaciones: genomic analysis
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• Clave InChI: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Descripción

Descripción general

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Aplicación

Formamide has been used:

• as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
• in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
• in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
• to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
• as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

La formamida desestabiliza los dúplex de ácidos nucleicos y puede utilizarse, normalmente, a una concentración del 50 % en protocolos de hibridación que requieran temperaturas de hibridación mas bajas.

Acciones bioquímicas o fisiológicas

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Características y beneficios

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Otras notas

For additional information on our range of Biochemicals, please complete this form.

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H351,H360D,H373
• Consejos de prudencia: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Órganos de actuación: Blood
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): 305.6 °F
• Punto de inflamabilidad (°C): 152 °C
• Equipo de protección personal: Eyeshields, Gloves, type ABEK (EN14387) respirator filter