Saltar al contenido
Merck

Saltar a

38733

Laemmli Lysis-buffer

non smelling

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
Talla/SKUDisponibilidadPrecio
5 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
44,20 €
5 x 2 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
81,60 €

Acerca de este artículo

NACRES:
NA.25
UNSPSC Code:
12161700

44,20 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Solicita un pedido personalizado
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle


Quality Segment

form

liquid

composition

bromophenol blue, 0.004% , DTT, 400 mM , glycerol, 20% , SDS, 4% , TRIS, 0.125 M

pH

~6.8

suitability

in accordance for electrophoresis test

storage temp.

−20°C

Application

Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
B5020B4895P2192
description

non smelling

description

pH 4.00±0.01 (25 °C)

description

pH 10.00±0.01 (25 °C)

description

Optimized for routine PCR with MgCl2 included

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

300

form

liquid

form

liquid

form

liquid

form

liquid

pH

~6.8

pH

4.00±0.01 (25 °C)

pH

10.00±0.01 (25 °C)

pH

-

storage temp.

−20°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

−20°C

suitability

in accordance for electrophoresis test

suitability

-

suitability

-

suitability

-

composition

bromophenol blue, 0.004% , DTT, 400 mM , glycerol, 20% , TRIS, 0.125 M , SDS, 4%

composition

-

composition

-

composition

-


pictograms

CorrosionExclamation mark

signalword

Danger

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B

Clase de almacenamiento

8B - Non-combustible corrosive hazardous materials



Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos



Preguntas

1–7 de 7 Preguntas  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      ¿Le ha resultado útil?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      ¿Le ha resultado útil?

  3. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    1 respuesta
    1. The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

      ¿Le ha resultado útil?

  4. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    1 respuesta
    1. For most applications, it is considered to be a 2X solution.

      ¿Le ha resultado útil?

  5. What is the difference between the two Laemmli buffers S3401 and 38733?

    1 respuesta
    1. Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

      ¿Le ha resultado útil?

  6. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    1 respuesta
    1. An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

      ¿Le ha resultado útil?

  7. What is the Department of Transportation shipping information for this product?

    1 respuesta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos