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I5502

Sigma-Aldrich

Isopropyl β-D-1-thiogalactopyranoside

≥99% (TLC)

Sinónimos:

IPTG, Isopropyl β-D-thiogalactoside

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About This Item

Fórmula empírica (notación de Hill):
C9H18O5S
Número de CAS:
367-93-1
Peso molecular:
238.30
Beilstein:
4631
Número CE:
206-703-0
Número MDL:
MFCD00063273
Código UNSPSC:
12352201
ID de la sustancia en PubChem:
24896069
NACRES:
NA.31

descripción

non-ionic

Nivel de calidad

200

Análisis

≥99% (TLC)

formulario

powder

técnicas

thin layer chromatography (TLC): suitable

impurezas

10.0-22.0% Dioxane

color

white to off-white

mp

120 -122  °C ((248 - 252 °F ))

solubilidad

water: 50 mg/mL, clear, colorless

temp. de almacenamiento

2-8°C

cadena SMILES

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

Clave InChI

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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Aplicación

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Otras notas

Non-metabolizable galactose analog.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Reconstitución

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Palabra de señalización

WarningDanger

Frases de peligro

H319 - H351 - H319 - H350

Clasificaciones de peligro

Carc. 1B - Eye Irrit. 2

Riesgos supl.

EUH019

Código de clase de almacenamiento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificados de análisis (COA)

Lot/Batch Number
0000287005
0000369115
0000361359
0000361354
0000195893

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Sigma-Aldrich

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Sigma-Aldrich

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