XGAL-RO

X-Gal

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

• Sinónimos: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside, bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Fórmula empírica (notación de Hill): C₁₄H₁₅BrClNO₆
• Número de CAS: 7240-90-6
• Peso molecular: 408.63
• Beilstein: 1402009
• Número MDL: MFCD00005666
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 329830810
• NACRES: NA.28

Propiedades

• descripción: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
• Nivel de calidad: 100
• Análisis: 99%
• formulario: solid
• mol peso: 408.63 g/mol
• envase: pkg of 1 g (10745740001); pkg of 100 mg (03117073001); pkg of 100 mg (11680293001); pkg of 2.5 g (10703729001); pkg of 250 mg (10651745001)
• fabricante / nombre comercial: Roche
• concentración: 20 mg/mL
• técnicas: activity assay: suitable
• color: white
• solubilidad: DMF: soluble; methanol: soluble
• aplicaciones: genomic analysis; life science and biopharma
• temp. de almacenamiento: 2-8°C
• cadena SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• Clave InChI: OPIFSICVWOWJMJ-AEOCFKNESA-N

Descripción

Descripción general

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Aplicación

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Calidad

Chromatographically homogeneous

Forma física

Crystals, white powder

Nota de preparación

Working solution: Stock solution:

• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
• A_{590 nm}: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable