E9379

Stains-All

~95% purity, powder

• Sinónimos: 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Fórmula empírica (notación de Hill): C₃₀H₂₇BrN₂S₂
• Número de CAS: 7423-31-6
• Peso molecular: 559.58
• Beilstein: 3865213
• Número CE: 231-047-7
• Número MDL: MFCD00078788
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24894694
• NACRES: NA.47

Propiedades

• Nombre del producto: Stains-All, ~95%
• Ensayo: ~95%
• Nivel de calidad: 200
• Formulario: powder
• técnicas: microbe id | staining: suitable
• solubilidad: chloroform: 40 mg (PLUS 4.0 ml)
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• Clave InChI: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Descripción

Aplicación

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Acciones bioquímicas o fisiológicas

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Precaución

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)