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B3883

Bile bovine

dried, unfractionated

Sinónimos:

Ox gall powder

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A ustedes/SKUDisponibilidadPrecio
25 g
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48,90 €
100 g
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143,00 €
500 g
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403,00 €

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Número CAS:
UNSPSC Code:
41106212
EC Number:
232-369-0
NACRES:
NA.85
MDL number:

48,90 €


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sterility

non-sterile

Quality Segment

form

dried, unfractionated

color

yellow-brown

solubility

water: soluble 50 mg/mL

application(s)

microbiology

General description

Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.

Application

Bile bovine has been used:
  • in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition
  • as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology
  • as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583

Biochem/physiol Actions

Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.

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Este artículo
B8381B875648305
form

dried, unfractionated

form

powder

form

powder

form

powder

application(s)

microbiology

application(s)

-

application(s)

microbiology

application(s)

microbiology

sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

solubility

water: soluble 50 mg/mL

solubility

-

solubility

distilled water: freely soluble

solubility

H2O: 0.1 g/mL, clear, faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

color

yellow-brown

color

-

color

-

color

-


Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Preguntas

1–4 de 4 Preguntas  
  1. Hi, I am trying to prepare blank bile matrix using this bovine bile powder. Do you have any recommendation of mixing ratio? Thank you

    1 respuesta
    1. This product is a crude bile extract from ox gall bladder. B3883 is soluble in water at 50 mg per mL resulting in a turbid dark brown to green suspension. The natural concentration is 1 gram of solid material with 11.5 grams of  water. We do not have more detailed information about using it in preparing a blank matrix.

      ¿Le ha resultado útil?

  2. WHAT IS THE PROPER TEMPERATURE FOR STORAGE?

    1 respuesta
    1. Item B3883 can be stored at room temperature.

      ¿Le ha resultado útil?

  3. Can you tell us the exact bile salt concentration?

    1 respuesta
    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.

      ¿Le ha resultado útil?

  4. I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.

    1 respuesta
    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.

      ¿Le ha resultado útil?

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