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Merck
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48305

Millipore

Bile salts

suitable for microbiology

Sinónimos:

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture, Bile acids sodium salt, Cholic acid-Deoxycholic acid sodium salt mixture

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About This Item

Número MDL:
MFCD00130648
Código UNSPSC:
41106212
NACRES:
NA.85

descripción

anionic

Nivel de calidad

200

esterilidad

non-sterile

Análisis

40-60% (Cholic Acid ; approximately 50% sodium cholate and approximately 50% sodium deoxycholate, HPLC)

formulario

powder

composición

cholic acid sodium salt, ~50%
deoxycholic acid sodium salt, ~50%

impurezas

≤5% water

pérdida

≤5% loss on drying (water)

solubilidad

H2O: 0.1 g/mL, clear, faintly yellow

aplicaciones

microbiology

temp. de almacenamiento

10-30°C

Categorías relacionadas

Descripción general

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.
Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Aplicación

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.

Acciones bioquímicas o fisiológicas

Bile salts are surface-active steroid compounds and their primary physiological function is to aid in the digestion of lipophilic nutrients in intestinal tracts of vertebrates.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCK4542
BCCH7185
BCCJ5649
BCCJ5529
BCCG0220

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Eunsil Choi et al.
Frontiers in microbiology, 11, 597515-597515 (2020-11-27)
The BipA (BPI-inducible protein A) protein is ubiquitously conserved in various bacterial species and belongs to the translational GTPase family. Interestingly, the function of Escherichia coli BipA is not essential for cell growth under normal growth conditions. However, cultivation of
Bacterial degradation of bile salts.
Philipp B
Applied Microbiology and Biotechnology, 89 (4), 903-915 (2011)
Bonggyu Min et al.
Frontiers in bioengineering and biotechnology, 8, 828-828 (2020-08-15)
Stress resistance is an important trait expected of lactic acid bacteria used in food manufacturing. Among the various sources of stress, high temperature is a key factor that interrupts bacterial growth. In this regards, constant efforts are made for the
Luis Noriega et al.
International journal of food microbiology, 94(1), 79-86 (2004-06-03)
Six derivatives with increased resistance to ox gall (MIC: > or = 1% w/v) and one derivative resistant to sodium cholate (MIC: 0.8% w/v) were obtained from more sensitive original Bifidobacterium strains. These microorganisms, and two additional cholate resistant derivatives
D L Scheusner et al.
Applied microbiology, 21(1), 46-49 (1971-01-01)
A population of Escherichia coli ML30 cells was exposed to a quaternary ammonium compound, and injury to the cells was measured by a comparison of counts on Trypticase Soy Agar and Violet Red Bile Agar. Substantial injury could not be
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Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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