Dextran is a D-glucosyl homopolysaccharide which has a-1,6 glucosidic linkages with different branches through α-1,2, α-1,3, and α-1,4 linkages correspondingly classified as group A, B and C. It can be synthesized from sucrose by cell-free filtrate.
Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, with a linear backbone of α-linked D-glucopyranosyl repeating units. Dextrans are found as bacterial extracellular polysaccharides. They are synthesized from sucrose by Leuconostoc mesenteroides and Lactobacillus brevis. Bacteria employ dextran in biofilm formation or as a protective coating to evade host phagocytes in the case of pathogenic bacteria. Dextran from Leuconostoc mesenteroides (Mw: 25,000) may be used as an analytical standard to calibrate the column for gel permeation chromatography (GPC).
Aplicación
El uso de dextranos como brazos espaciadores largos e hidrófilos mejora el rendimiento de las proteínas inmovilizadas que actúan sobre las macromoléculas.
Markers-dextran with 25000 Da has been used as standard in gel chromatography analysis to determine fractional composition of protein, carbohydrate, phenolic surface active substances in base wine.
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Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, featuring a linear backbone of α-linked d-glucopyranosyl repeating units.
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