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206865

Sigma-Aldrich

Primuline

Dye content 50 %

Sinónimos:

Direct Yellow 59, Primuline Yellow

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About This Item

Fórmula empírica (notación de Hill):
C21H14N3NaO3S3
Número de CAS:
8064-60-6
Peso molecular:
475.54
Número de índice de color:
49000
Número MDL:
MFCD00005798
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24852417
NACRES:
NA.47

formulario

powder or crystals

Nivel de calidad

100

composición

Dye content, 50%

mp

>300 °C

λmáx.

229 nm (2nd)
340-355 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

[Na+].Cc1ccc2nc(sc2c1S([O-])(=O)=O)-c3ccc4nc(sc4c3)-c5ccc(N)cc5

InChI

1S/C21H15N3O3S3.Na/c1-11-2-8-16-18(19(11)30(25,26)27)29-21(24-16)13-5-9-15-17(10-13)28-20(23-15)12-3-6-14(22)7-4-12;/h2-10H,22H2,1H3,(H,25,26,27);/q;+1/p-1

Clave InChI

RSRNHSYYBLEMOI-UHFFFAOYSA-M

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Aplicación

Primuline has been used as a reagent in the preparation of lipids for chloroplast lipid membrane. It has been used as a dye in preparative thin-layer chromatography (TLC).

Acciones bioquímicas o fisiológicas

Primuline, also known as direct yellow 59 or primuline yellow, is considered to be a more sensitive and non-destructive dye for lipids. It is widely used as a fluorescent dye in preparative thin-layer chromatography (TLC). Primuline is suitable for vital staining. It is used as a fluorochrome in plant tissues. Primuline is also used to stain pollen grains and lignified cell walls. In addition, it also helps in segregating intact and broken starch grains. At pH 8, primuline acts as a fluorochrome for mast cells.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SHBR3189
SHBP1887
SHBM2490
MKCH8128
MKBT2168V

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J I Morrell et al.
Science (New York, N.Y.), 217(4566), 1273-1276 (1982-09-24)
A method combining steroid autoradiography and fluorescent dye retrograde neuroanatomical tracing has been devised. This method makes it possible to demonstrate that some estrogen-concentrating cells in the ventrolateral subdivision of the ventromedial nucleus of the rat hypothalamus are neurons that
[Dopamine-containing neurons retrogradely labeled with primuline--a source of descending supraspinal fibers in the rat brain].
F N Serkov et al.
Doklady Akademii nauk SSSR, 292(6), 1506-1509 (1987-01-01)
SEQUENTIAL SYNTHESIS AND METHYLATION OF
PHOSPHATIDYLETHANOLAMINE PROMOTES LIPID DROPLET BIOSYNTHESIS
AND STABILITY IN TISSUE CULTURE AND IN VIVO.
Horl G
The Journal of Biological Chemistry (2011)
T Taki et al.
Analytical biochemistry, 223(2), 232-238 (1994-12-01)
A new and simple method for purifying glycosphingolipids and phospholipids by using "TLC blotting" was established. Glycosphingolipids separated by two-dimensional thin-layer chromatography (TLC) were made visible with primuline reagent, and then bands were marked with a drawing colored pencil. The
J L Labandeira Garcia et al.
Journal of anatomy, 137 (Pt 2), 247-261 (1983-09-01)
This paper describes a qualitative and quantitative investigation into the location of the motoneurons innervating the extra-ocular muscles of the rat. Injections of horseradish peroxidase, bisbenzimide, propidium iodide and DAPI-primuline were made either in one or simultaneously in two muscles.
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