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198161

Sigma-Aldrich

Toluidine Blue O

certified by the Biological Stain Commission

Sinónimos:

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Fórmula empírica (notación de Hill):
C15H16ClN3S
Número de CAS:
92-31-9
Peso molecular:
305.83
Número de índice de color:
52040
Número CE:
202-146-2
Número MDL:
MFCD00011934
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24851865
NACRES:
NA.47

grado

certified by the Biological Stain Commission

Nivel de calidad

200

formulario

powder

composición

Dye content, 80%

color

dark green

λmáx.

626 nm

ε (coeficiente de extinción)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

Clave InChI

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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Descripción general

A metachromatic dye suitable for a wide variety of histological staining procedures.

Aplicación

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Acciones bioquímicas o fisiológicas

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Idoneidad

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SHBQ4530
SHBR8115
SHBP9405
SHBQ3835
SHBP2785

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The Effect of Variable Energy Input from a Novel Light Source on the Photoactivated Bactericidal Action of Toluidine Blue O on Streptococcus mutans
Williams J A
Caries Research, 37, 190?193-190?193 (2003)
Polychromatic staining of plant cell walls by toluidine blue O
Brien T P O
Protoplasma, 59(2), 368- 373 (1964)
Anaīs Noblanc et al.
Antioxidants (Basel, Switzerland), 9(2) (2020-02-15)
There is growing evidence that the quality of spermatozoa decreases with age and that children of older fathers have a higher incidence of birth defects and genetic mutations. The free radical theory of aging proposes that changes with aging are
Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode.
Zanin IC
European Journal of Oral Sciences, 114(1), 64-69 (2006)
Catherine White
Best practice & research. Clinical obstetrics & gynaecology, 27(1), 113-130 (2012-12-12)
The examination of the rape victim should focus on the therapeutic, forensic and psychological needs of the individual patient. One aspect will be an examination for ano-genital injuries. From a medical perspective, they tend to be minor and require little
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