usage
sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001)
manufacturer/tradename
Roche
parameter
37 °C optimum reaction temp.
storage temp.
−20°C
General description
The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Application
The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
- Cloning of fragments into either plasmid or phage vectors[1]
- Linker ligation
- Recircularization of linear vector DNA
- Generation of libraries
Biochem/physiol Actions
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Packaging
1 kit containing 5 components
Other Notes
For life science research only. Not for use in diagnostic procedures.
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
1 of 1
Este artículo | |||
|---|---|---|---|
| usage sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001) | usage sufficient for 40 reactions (DNA ligation) | usage kit sufficient for 50 ligation reactions | usage - |
| parameter 37 °C optimum reaction temp. | parameter - | parameter - | parameter 4-22 °C optimum reaction temp. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche |
Solo componentes del kit
Referencia del producto
Descripción
- rAPid Alkaline Phosphatase Buffer 10x concentrated
- rAPid Alkaline Phosphatase 1 U/μl
- DNA Dilution Buffer 5x concentrated
- T4 DNA Ligation Buffer 2x concentrated
- T4 DNA Ligase 5 U/μl
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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