11759779001
Roche
BM Chemiluminescence ELISA Substrate (AP)
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About This Item
Código UNSPSC:
12352204
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uso
sufficient for 1,000 tests (ELISA)
sufficient for 600 tests (SEAP)
envase
pkg of 150 mL
fabricante / nombre comercial
Roche
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Especificidad
The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).
Características y beneficios
- Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
- High dynamic measuring range: Linear range of more than five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics
Componentes
1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer
2. Enhancer, Emerald II
3. Assay Buffer
Principio
CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.
Nota de preparación
Working solution: Preparation of working solution
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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