11138600001

Interleukin-6, human (hIL-6)

recombinant (E. coli)

• Sinónimos: hIL-6, interleukin-6 human
• Código UNSPSC: 12352207

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in E. coli
• Análisis: >98% (SDS-PAGE)
• formulario: solution
• potencia: <0.01 ng/mL EC₅₀
• mol peso: 20,600 Da
• envase: pkg of 200,000 U (2 μg, 1 ml)
• fabricante / nombre comercial: Roche
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | hybridoma: suitable
• impurezas: <0.1 EU/μgtested (LAL test)
• Nº de acceso UniProt: P05231
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL6(3569)

Descripción

Descripción general

Interleukin-6, human (hIL-6), is produced in E. coli and purified by standard chromatographic techniques.

Especificidad

Recombinant IL-6, human is effective on mouse and human cells.
IL-6, a 26 kD protein, also known as B-cell stimulatory factor 2 (BSF-2, BCDF), interferon-β₂ (IFN-β₂), hybridoma/plasmacytoma growth factor (H(P)GF), and hepatocyte-stimulating factor is produced by many cell types (e.g., T-cells, monocytes, fibroblasts, endothelial cells). IL-6 induces the terminal maturation of activated B-cells into antibody-producing cells (B-cell differentiation activity. The hepatic acute phase response is induced by IL-6 (hepatocyte-stimulating activity). It was shown that IL-6 and IL-3 act synergistically to support the proliferation of hematopoietic stem cells (hemapoietic activity), however, IL-6 does not have antiviral activity, although it has been called IFN-β₂. High affinity receptors for IL-6 are found on many cell types.

Aplicación

Recombinant IL-6, human, is a growth factor for murine and human B-cells. It can be used to replace feeder cells in the preparation of murine and human hybridomas.

Acciones bioquímicas o fisiológicas

Interleukin-6 participates in several pleiotropic functions in various types of cells through its specific receptor (IL-6-R). It has been suggested that interleukin-6 may participate in the interaction between neuroendocrine and immune system by activating adrenocorticotropic hormone secretion. It has also been reported that IL-6 stimulates the bonding of receptor (IL-6-R) with a non-ligand-binding membrane glycoprotein, gp130.

Calidad

Purity: 95% pure as determined by HPLC or SDS-PAGE [Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test).
Note: 1 EU corresponds to 0.1 ng

Secuencia

Chain Length 184 AA

The primary structure of recombinant human IL-6 is identical to that of natural human IL-6 (one polypeptide chain, 184 amino acids), however, it contains an altered sequence within the first 15 amino-terminal amino acids.

Definición de unidad

EC₅₀ definition: The amount of hIL-6 that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 7TD1 cells (1 unit equals <0.01 ng).

Forma física

Solution, filtered through 0.2 μm pore size membrane.

Nota de preparación

Working concentration: 10 to 100 U/ml (0.1 1 ng/ml)
10 to 100 U/ml (0.1 to 1 ng/ml)
A concentration of 100 U/ml (1 ng/ml) is recommended for the preparation of B-cell hybridomas
Working solution: Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Reconstitución

Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.

Nota de análisis

Specific activity/EC₅₀: >1 x 10⁸ U/mg, <0.01 ng/ml (hIL-6, NIBSC, interim standard, 88/514), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash