11011375001
Roche
Nutridoma™-SP
suitable for hybridoma, solution, pkg of 100 mL (100x)
Sinónimos:
hybridoma, nutridoma
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About This Item
Código UNSPSC:
12352207
Productos recomendados
esterilidad
sterile; 0.2 μm filtered
Nivel de calidad
formulario
solution
envase
pkg of 100 mL (100x)
fabricante / nombre comercial
Roche
concentración
100 ×
técnicas
cell culture | hybridoma: suitable
impurezas
mycoplasma, tested
≤100 EU/mL Endotoxin (LAL test)
solubilidad
water: miscible
temp. de almacenamiento
20-25°C
Categorías relacionadas
Descripción general
Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells. It is used for the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.
Aplicación
Nutridoma-SP has been used in the culture media for canine trophoblasts, human embryonic stem cells and PLB-985 cells.
Definición de unidad
Endotoxin (LAL): 10 EU/ml; mycoplasma-tested
Forma física
Solution, 100x concentrated, filtered through 0.2 μm pore size membrane
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Nota de preparación
Working concentration: Nutridoma-SP (100x) is diluted 1:100 (v/v) with sterile basal medium. Use high glucose DMEM/RPMI 1640 (1:1) (R/D medium) for Nutridoma-SP. The final medium should also contain L-glutamine and sodium bicarbonate. The protein concentration is less than 50 μg/ml for 1% working concentration.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Información legal
Nutridoma is a trademark of Roche
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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