The information provided by the supplier for SCT117 indicates that the product is at a 200x concentration. To perform staining of fixed and permeabilized cells for cell number normalization, it is recommended to conduct a standard curve of cell density and test RedDot™1 at 1X and 2X final concentration in order to determine the optimal staining concentration for achieving a linear fluorescence signal for your specific cell type.
SCT117
BioTracker NIR694 Nuclear Dye (Water)
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Sinónimos:
Live cell imaging probe
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250 μL
357,00 €
357,00 €
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250 μL
357,00 €
About This Item
Código UNSPSC:
12352207
NACRES:
NA.47
357,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Productos recomendados
técnicas
cell based assay: suitable
método de detección
fluorometric
Condiciones de envío
ambient
Descripción general
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Aplicación
Live cell fluorescent imaging
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Calidad
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Absorbance: 662nm
Emission: 694nm
Forma física
Liquid
Almacenamiento y estabilidad
Store BioTracker NIR694 Nuclear Dye (Water) at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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The data sheet indicates to dilute BioTracker™ NIR694 Nuclear Dye in cell culture medium or PBS to a final concentration of 1X. However, it does not specify the starting concentration, leaving uncertainty about the appropriate dilution ratio. Can you provide guidance on the starting concentration and the amount needed for dilution?
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What is the stock concentration of this product? We purchased it recently, but it doesn’t say on the website or the product documentation.
1 respuesta-
SCT117 is provided at a 200X concentration.
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Filtros activos
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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