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SCM112

Sigma-Aldrich

3dGRO® Wnt3a Conditioned Media Supplement

Sinónimos:

Wnt3a Conditioned Media;Wnt3a CM;Wnt Conditioned Media

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.76

envase

1 ea of 50 mL

fabricante / nombre comercial

Millipore

técnicas

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción línea celular

3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO® Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.SourceThe 3dGRO® Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.

Aplicación

  • Appearance (color): clear, red liquid
  • Endotoxin: 2 EU/mL
  • Sterility Tested: No Growth/Pass
  • Mycoplasma: Negative
  • Media tested for function in supporting growth of human pancreatic organoids
  • Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells

Características y beneficios

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

Almacenamiento y estabilidad

Wnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.

Información legal

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Human Pancreatic Organoids for Cancer Research Applications

Human pancreatic cancer organoid biobank (PDAC organoids) with various KRAS mutations to aide in 3D cell culture and cancer research applications.

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