MABT828

Anti-Myoferlin Antibody, clone 159

culture supernatant, clone 159, from mouse

• Sinónimos: Myoferlin, Fer-1-like protein 3
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: culture supernatant
• tipo de anticuerpo: primary antibodies
• clon: 159, monoclonal
• reactividad de especies: mouse, human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_038479
• Nº de acceso UniProt: Q9NZM1
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MYOF(26509)

Descripción

Descripción general

Myoferlin (UniProt Q9NZM1; also known as Fer-1-like protein 3) is encoded by the MYOF (also known as FER1L3, KIAA1207) gene (Gene ID 26509) in human. Myoferlin is a calcium/phospholipid-binding protein that is expressed in myoblast and endothelial cells and in cardiac and skeletal muscles. Low levels of myoferlin are seen in lung, and very low levels in kidney, placenta, and brain. Myoferlin plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Myoferlin contains C2 domains that participate in calcium-mediated membrane fusion events. It is also involved in endocytic recycling and has been implicated in VEGF signaling by regulating the levels of the receptor KDR. Myoferlin is overexpressed in breast cancer cells and its depletion blocks EGF-induced cell migration and epithelial to mesenchymal transition and reduces invasion through extracellular matrix (ECM). Ref.: Turtoi, A., et al. (2013). Cancer Res. 73, 5438-5448.

Especificidad

Antibody blocking with the immunogen peptide prior to Western blotting abolished the target band detection (Jaiswal, J.K., et al. (2007). Traffic. 8(1):77-88). Epitope is absent from human isoform 4 and mouse isoform 3, but present in all other human and mouse spliced isoforms reported by UniProt (Q9NZM1 and Q69ZN7).

Inmunógeno

KLH-conjugated linear peptide corresponding to a sequence within the region between the second and the third C2 domain of human myoferlin.

Aplicación

Immunocytochemistry Analysis: The undiluted hybridoma culture supernatant from a representative lot detected myoferlin in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts.

Western Blotting Analysis: A representative lot detected myoferlin in C2C12 murine myoblast lysate and human skin fibroblast lysate. Antibody blocking with the immunogen peptide prior to Western blotting abolished the target band detection (Jaiswal, J.K., et al. (2007). Traffic. 8(1):77-88).

Research Category
Cell Structure

This mouse monoclonal hybridoma culture supernatant, Anti-Myoferlin, clone 159, Cat. No. MABT828, is validated for use in Immunocytochemistry and Western Blotting for the detection of Myoferlin.

Calidad

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:10 dilution of this hybridoma culture supernatant detected myoferlin isoforms in 10 µg of C2C12 murine myoblast lysate.

Descripción de destino

~233/207/103 kDa observed. 234.7/229.9/233.3/179.6/233.5/49.72/46.70 kDa (human isoform 1/2/3/5/6/7/8), 233.3/103.4/207.6 kDa (mouse isoform 1/2/4) calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Mouse IgG2aκ hybridoma culture supernatant without azide.

Unpurified.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2