MABE2037

Anti-STAG1/SA1 Antibody, clone SUSI63B

• Sinónimos: Cohesin subunit SA-1 (UniProt: Q9D3E6;also known as SCC3 homolog 1;Stromal antigen 1
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: SUSI63B, monoclonal
• mol peso: calculated mol wt 144.44 kDa; observed mol wt ~145 kDa
• purificado por: using protein G
• reactividad de especies: human, mouse
• envase: antibody small pack of 100 μL
• técnicas: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NP_033308
• Nº de acceso UniProt: Q9D3E6
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Pnpla3(116939)

Descripción

Descripción general

Cohesin subunit SA-1 (UniProt: Q9D3E6; also known as SCC3 homolog 1, Stromal antigen 1) is encoded by the Stag1 (also known as Sa1) gene (Gene ID: 20842) in murine species. Cohesin are known to mediate sister chromatid cohesion and 3D genome folding. They hold the sister chromatids together to ensure correct DNA repair by homologous recombination and proper chromosome segregation during cell division. They are essential for cell proliferation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped and at anaphase stage. The complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. This complex may also play a role in spindle pole assembly during mitosis. Two variant cohesin complexes containing Structural Maintenance of Chromosomes (SMC) 1, SMC3, RAD21 and either STAG/SA1 or STAG2/SA2 are present in all cell types. Before prophase, STAG1/SA1 appears to be scattered along chromosome arms. However, during prophase, most of cohesin complexes dissociate from chromatin. This dissociation is probably due to its phosphorylation by PLK1. At anaphase, the RAD21 subunit is cleaved that leads to the dissociation of the complex from chromosomes, allowing their separation. (Ref.: De Koninck, M., et al. (2020). Cell Rep.32(6); 108014; Kojic, A., et al. (2018). Nat. Struct. Mol. Biol. 25(6); 496-504).

Especificidad

Clone SUSI63B is a rat monoclonal antibody that detects STAG1/SA1. It targets an epitope within the N-terminal region.

Inmunógeno

MBP-tagged recombinant fragment corresponding to the first 225 amino acids from N-terminal region of mouse Cohesin subunit SA-1 (STAG1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.

Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).

Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1