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MABD117

Sigma-Aldrich

Anti-GATA Antibody3, clone 7B5

ascites fluid, clone 7B5, from mouse

Sinónimos:

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

clon:
7B5, monoclonal
application:
IHC
WB
reactividad de especies:
human
técnicas:
immunohistochemistry: suitable
western blot: suitable
citations:

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

7B5, monoclonal

reactividad de especies

human

técnicas

immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

wet ice

Descripción general

GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.

Inmunógeno

Purified recombinant fragment of human GATA3 expressed in E. Coli.

Aplicación

Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Calidad

Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.

Descripción de destino

~75 kDa observed

Forma física

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
GATA3-hIgGFc transfected HEK293 cell lysate

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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