MAB8887

Anti-Collagen Type II Antibody, clone 6B3

clone 6B3, Chemicon^®, from mouse

• Sinónimos: Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 6B3, monoclonal
• reactividad de especies: human, chicken, mouse, salamander
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_001844.4; NM_033150.2
• Nº de acceso UniProt: P02458
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... COL2A1(1280)

Descripción

Especificidad

In Western blotting (but not ELISA), MAB8887 recognizes both a1(II) and a3(XI) chains which have identical primary structure. In Western blotting, MAB8887 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. It will not react with intact Collagen II under traditional conditions.

Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.

In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.

Inmunógeno

Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.

Aplicación

Anti-Collagen Type II Antibody, clone 6B3 is an antibody against Collagen Type II for use in IF, IH & WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot (Mayne, 1994)

Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.

Immunofluorescence

ELISA

Optimal working dilutions must be determined by end user.

Descripción de destino

120 kDa

Forma física

Format: Purified

Protein G Purified

Purified immunoglobulin in 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Almacenamiento y estabilidad

Maintain at 2–8°C in undiluted aliquots for up to 12 months

Nota de análisis

Control
Cartilage in lung or fetus

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable