MAB19101

Anti-Tenascin Antibody, clone DB7

clone DB7, Chemicon^®, from mouse

• Sinónimos: Tenascin-C
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: DB7, monoclonal
• reactividad de especies: human, rabbit, guinea pig
• no debe reaccionar con: rat, mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG2a
• Nº de acceso UniProt: P24821
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TNC(3371)

Descripción

Especificidad

The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein

Inmunógeno

Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.

Aplicación

Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).

Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.

Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.

Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.

Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)

Final dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Ligadura / enlace

Replaces: MAB1902

Forma física

Format: Purified

The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8°C

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable