MAB1397Z

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

clone 3E2B, Chemicon^®, from hamster(Armenian)

• Sinónimos: CD54, MAB1397
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: hamster (Armenian)
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3E2B, monoclonal
• reactividad de especies: mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_000201.1
• Nº de acceso UniProt: P05362
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Icam1(15894)

Descripción

Especificidad

MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.

Aplicación

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.

Immunocytochemistry: Live or acetone fixed cells

ELISA

Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

Clone 3E2B is reported to be function blocking against murine ICAM-1.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Ligadura / enlace

Replaces: CBL1331F

Forma física

Format: Purified

Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg

Almacenamiento y estabilidad

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable