IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Sinónimos:
Affinity resin, Protein G/Agarose
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About This Item
Código UNSPSC:
41116133
NACRES:
NA.56
Productos recomendados
formulario
slurry (Liquid)
contiene
≤0.1% sodium azide as preservative
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
do not freeze
técnicas
protein purification: suitable
idoneidad
suitable for microbiology
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Aplicación
Immunoprecipitation (see comments)
Advertencia
Toxicity: Standard Handling (A)
Forma física
33% slurry in PBS.
Otras notas
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
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