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PureProteome Protein G Magnetic Bead System

The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability

Sinónimos:

Protein G Magnetic Beads, PureProteome Magnetic Beads

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Tamaño de envaseSKUDisponibilidadPrecio
2 x 1 mL
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319,00 €
10 mL
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1490,00 €

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UNSPSC Code:
41105507
NACRES:
NA.56
eCl@ss:
32160405

319,00 €


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packaging

pkg of 2 × 1 mL

manufacturer/tradename

PureProteome

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

particle size

10 μm

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

shipped in

wet ice

General description

The PureProteome Protein G Magnetic Beads contain Protein G, a binding protein for antibodies, which is coated on the surface of the beads. These beads are designed to isolate immunoglobulins (IgG) from complex mixtures through antibody-affinity purification. These beads are used in conjunction with a magnetic stand to immobilize the beads. Following this step, the sample is washed to remove any impurities, and then the immobilized IgG can be eluted for further analysis or experimentation.

Application

PureProteome Protein G Magnetic Bead System is suitable for:
  • serum depletion
  • immunoprecipitation
  • protein purification    

PureProteome Protein G Magnetic Bead System is suitable:

  • for isolation of the immunoglobulin G (IgG) fraction from a small volume of plasma
  • for co-immunoprecipitation (Co-IP)

Features and Benefits

  • PureProteome Magnetic Beads are very well suited for fully automated purification process using the KingFisher Duo particle processor, it is not dependent on the cell lysate clarification step
  • The entire process is reproducible, and the results are comparable to that of a standard protocol using the PureProteome Magnetic Stand
  • PureProteome Magnetic Beads is compatible with any automated system for low, medium, or high-throughput sample preparation.

Legal Information

PureProteome is a trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
LSKMAGALSKMAGHLSKMAGL10
manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

particle size

10 μm

particle size

10 μm

particle size

10 μm

particle size

10 μm

packaging

pkg of 2 × 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 10 mL

packaging

pkg of 10 mL

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1-5.5 mg/mL, bead suspension binding capacity (protein)

capacity

-

technique(s)

depletion: suitable (serum), protein purification: suitable, immunoprecipitation (IP): suitable

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

protein purification: suitable (histidine tagged recombinant protein)

technique(s)

depletion: suitable (serum), protein purification: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice


Clase de almacenamiento

12 - Non Combustible Liquids

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Contenido relacionado

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

Purification of recombinant proteins expressed in E.coli requires many time-consuming steps. To liberate the protein of interest, traditional bacterial lysis relies on the addition of lysozyme and a combination of sonication and repeated freeze/thaw cycles to break the bacterial cell wall. Disruption of the cell is accompanied by an increase in the viscosity of the suspension, due to the release of DNA. An endonuclease is added to digest the DNA, thus reducing the viscosity of the lysate. Finally, to render the lysate compatible with traditional purification methods, insoluble cell debris must be removed by centrifugation.

Protein Blotting Survival Handbook: Tips and Tricks

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SKUGTIN
LSKMAGG1004053252323188
LSKMAGG0204053252373053

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