CC077

Human Collagen Type V

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• Sinónimos: Collagen Type V
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Propiedades

• Nombre del producto: Human Collagen Type V,
• origen biológico: human
• Nivel de calidad: 100
• Ensayo: 95% (SDS-PAGE)
• Formulario: liquid
• fabricante / nombre comercial: Chemicon^®
• concentración: 1 mg/mL
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.5% non-collagen proteins; <1% collagen type III; <2% collagen type I; <2% collagen type IV
• entrada: sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s); sample type epithelial cells; sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type: human embryonic stem cell(s)
• Nº de acceso NCBI: NM_000093.3
• Nº de acceso UniProt: P20908
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... COL5A1(1289) , COL5A2(1290)

Descripción

Descripción general

Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen.[1] It is minor collagen present in the extracellular matrix.[2]

Aplicación

Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR).[3] It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.[1]

Acciones bioquímicas o fisiológicas

Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.[2]

Forma física

Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.

Nota de preparación

Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.

Otras notas

Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable