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Merck

CC065

Human Tenascin

from human glioma cell line (U251), liquid, 0.1 mg/mL, suitable for cell culture

Sinónimos:

TN-C, Tenascin, Hexabrachion, Cytotactin, Neuronectin

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UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.1 mg/mL
Mol wt:
280—300 kDa
Assay:
>97% (SDS-PAGE)

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Nombre del producto

Human Tenascin-C Purified Protein,

biological source

human

Quality Level

assay

>97% (SDS-PAGE)

form

liquid

mol wt

280—300 kDa

manufacturer/tradename

Chemicon®

concentration

0.1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)

solubility

water: soluble

NCBI accession no.

UniProt accession no.

binding specificity

Peptide Source: Fibrinogen

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TNC(3371)

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Este artículo
F135CC115SRP8011
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

biological source

human

biological source

human

biological source

chicken

biological source

human

concentration

0.1 mg/mL

concentration

-

concentration

0.755 mg/mL

concentration

≥0.2 mg/mL

assay

>97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

-

assay

>90% (SDS-PAGE)

Quality Level

100, 300

Quality Level

200

Quality Level

100

Quality Level

-

mol wt

280—300 kDa

mol wt

~90 kDa

mol wt

large molecular weight polypeptide 190 kDa, large molecular weight polypeptide 200 kDa, large molecular weight polypeptide 220 kDa

mol wt

~10 kDa by SDS-PAGE

General description

Product Source: Human glioma cell line (U251).
Purified Human Tenascin-C Protien. Tenascin is known to: 1) play an active role in development of central nervous system and mesenchymal-derived organs; 2) exist in vasculature of tumors in adults; and 3) function in cell adhesion. By SDS-PAGE analysis, the protein migrates around 280-300kD.

Application

Cell adhesion assays.

Optimal working dilutions must be determined by end user.

Physical form

Purified protein supplied as a liquid in PBS containing no preservatives.

Preparation Note

Maintain at -70ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Replaces: CC066

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Longui Ning et al.
BioMed research international, 2019, 1475705-1475705 (2019-12-31)
Tenascin-C (TNC) is an extracellular matrix glycoprotein expressed in response to inflammation and tissue damage. The role of TNC in patients with inflammatory bowel disease (IBD) is not well understood. In this study, we analyzed the expression of TNC in
So-Young Yeo et al.
Nature communications, 9(1), 3016-3016 (2018-08-03)
Although fibroblasts are dormant in normal tissue, they exhibit explosive activation during wound healing and perpetual activation in pathologic fibrosis and cancer stroma. The key regulatory network controlling these fibroblast dynamics is still unknown. Here, we report that Twist1, a
Susobhan Sarkar et al.
Cancer research, 77(12), 3231-3243 (2017-04-19)
Oncogenic signaling by NOTCH is elevated in brain tumor-initiating cells (BTIC) in malignant glioma, but the mechanism of its activation is unknown. Here we provide evidence that tenascin-C (TNC), an extracellular matrix protein prominent in malignant glioma, increases NOTCH activity
Azhar Maqbool et al.
World journal of cardiology, 8(5), 340-350 (2016-05-28)
To investigate the effect of Tenascin C (TNC) on the expression of pro-inflammatory cytokines and matrix metalloproteinases in human cardiac myofibroblasts (CMF). CMF were isolated and cultured from patients undergoing coronary artery bypass grafting. Cultured cells were treated with either
Jian Cao et al.
Cell reports, 6(5), 868-877 (2014-03-04)
Metastasis is a major clinical challenge for cancer treatment. Emerging evidence suggests that aberrant epigenetic modifications contribute significantly to tumor formation and progression. However, the drivers and roles of such epigenetic changes in tumor metastasis are still poorly understood. Using

Protocolos

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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SKUGTIN
CC06504053252671982

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