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CBL154

Sigma-Aldrich

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon®, from mouse

Sinónimos:

CD44

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

F10-44-2, monoclonal

reactividad de especies

human

fabricante / nombre comercial

Chemicon®

técnicas

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotipo

IgG2a

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CD44(960)

Aplicación

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Forma física

Format: Purified

Nota de análisis

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Larissa M Macedo et al.
Journal of cellular physiology, 236(1), 366-378 (2020-06-11)
The effects of the renin-angiotensin system (RAS) on stem cells isolated from human dental apical papilla (SCAPs) are completely unknown. Therefore, the aim of this study was to identify RAS components expressed in SCAPs and the effects of angiotensin (Ang)
Arantxa Blázquez-Prunera et al.
Stem cell research & therapy, 8(1), 103-103 (2017-04-30)
Mesenchymal stem cells (MSCs) show promising characteristics for their use in advanced therapy medicinal products. However, there are some unresolved concerns, such as the use of animal components for their expansion. In this study we assessed the suitability of a
José M Díez et al.
Stem cell research & therapy, 6, 28-28 (2015-04-19)
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable.
Giulia Detela et al.
Biotechnology journal, 13(2) (2018-01-16)
Human mesenchymal stromal cells (hMSCs) are excellent candidates for cell therapy but their expansion to desired clinical quantities can be compromised by ex vivo processing, due to differences between donor material and process variation. The aim of this article is

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Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocolos

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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