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Sigma-Aldrich

Donkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed

Chemicon®, from donkey

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

origen biológico

donkey

Nivel de calidad

conjugado

TRITC conjugate
rhodamine conjugate

forma del anticuerpo

F(ab′)2 fragment of affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

reactividad de especies

rabbit

fabricante / nombre comercial

Chemicon®

técnicas

immunofluorescence: suitable

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Especificidad

Rabbit IgG

WAVELENGTH:
Absorption peak = 550nm, Emission peak = 570 nm.

Aplicación

Donkey anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by end user.

Forma física

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.

RHODAMINE/PROTEIN:
Approximately 0.52 (A550/A280)

Almacenamiento y estabilidad

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Aquatic Chronic 3

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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