Spastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.
Inmunógeno
Recombinant protein corresponding to human Spastin with 4 isoforms.
Aplicación
Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.
Western Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).
Calidad
Evaluated by Western Blotting in L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 µg of L6 cell lysate.
Descripción de destino
~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Axon regeneration is abortive in the central nervous system following injury. Orchestrating microtubule dynamics has emerged as a promising approach to improve axonal regeneration. The microtubule severing enzyme spastin is essential for axonal development and regeneration through remodeling of microtubule
The Journal of cell biology, 218(8), 2583-2599 (2019-06-23)
Lipid droplets (LDs) are neutral lipid storage organelles that transfer lipids to various organelles including peroxisomes. Here, we show that the hereditary spastic paraplegia protein M1 Spastin, a membrane-bound AAA ATPase found on LDs, coordinates fatty acid (FA) trafficking from
Preguntas
Revisiones
★★★★★ Sin puntuación
Filtros activos
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
