ABN34

Anti-PDE11A Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Dual 3′,5′-cyclic-AMP and -GMP phosphodiesterase 11A, cAMP and cGMP phosphodiesterase 11A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• técnicas: immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_058649
• Nº de acceso UniProt: Q9HCR9
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PDE11A(50940)

Descripción

Descripción general

The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5’-AMP and 5’GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome.

Especificidad

This antibody recognizes GAF domain of PDE11A.

Inmunógeno

Epitope: GAF domain.

KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A.

Aplicación

Detect PDE11A using this Anti-PDE11A Antibody validated for use in WB, IH.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE11A in normal mouse midbrain tissue.

Research Category
Neuroscience

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Calidad

Evaluated by Western Blot in human hippocampus tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PDE11A in 10 µg of human hippocampus tissue lysate.

Descripción de destino

~104 kDa observed. Uniprot states four isoforms at a molecular weight of ~105 kDa, ~78 kDa, ~66 kDa, and ~56 kDa This antibody can only recognize the ~105 kDa and the ~78 kDa isoforms.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human hippocampus tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids