ABE555

Anti-WTAP Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, hFL(2)D, Wilms tumor 1-associating protein, WT1-associated protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, human, mouse
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_001257460
• Nº de acceso UniProt: Q15007
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... WTAP(9589)

Descripción

Descripción general

Pre-mRNA-splicing regulator WTAP (UniProt Q15007; also known as Female-lethal (2)D homolog, hFL(2)D, Wilms tumor 1-associating protein, WT1-associated protein) is encoded by the WTAP (also known as KIAA0105) gene (Gene ID 9589) in human. WTAP is an ubiquitously expressed nuclear protein that is localized throughout the nucleoplasm and in speckles and partially co-localizes with splicing factors. A mouse homolog has also been reported that shares 96% sequence identity. WTAP regulates G2/M cell-cycle transition by binding to the 3′ UTR of CCNA2, which enhances its stability. WTAP knockdown is shown to induce G2 accumulation, which is partially rescued by adenoviral overexpression of cyclin A2. WTAP-null mice exhibit proliferative failure with early embryonic death. WTAP is also reported to be an oncogenic protein in acute myeloid leukemia (AML) and its increased expression has been linked to chemoresistance in AML. Ref: Horiuchi, K., et al. (2006), Proc. Natl. Acad. Sci. USA 103, 17278-17283. Bansal, H., et al. (2014). Leukemia 28, 1171-1174.

Especificidad

This polyclonal antibody targets an internal sequence within the N-terminal half of human WTAP.

Inmunógeno

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human WTAP.

Aplicación

Anti-WTAP, Cat. No. ABE555, is a highly specific rabbit polyclonal antibody that targets WTAP and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in A431.

Western Blotting Analysis: A 1:250 dilution of this antibody detected WTAP in 10 µg of A431 cell lysate.

Descripción de destino

~52 observed. 44.24/17.80 kDa (isoform 1/2 calculated). Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable