ABE184

Anti-Fox2 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: RNA binding protein fox-1 homolog 2, Fox-1 homolog B, Fox-1 homolog Fxh, Hexaribonucleotide-binding protein 2, RNA-binding motif protein 9, RNA-binding protein 9
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, rat, mouse
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_444334
• Nº de acceso UniProt: Q8BP71
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RBFOX2(23543)

Descripción

Descripción general

Fox2, also referred to as RNA binding protein fox-1 homolog 2, is an RNA binding protein involved in the regulation of tissue-specific alternative splicing. This protein interacts with the ER-alpha N-terminal activation domain, and contains a highly conserved RRM (RNA recognition motif), which associates with 5′-UGCAUGU-3′ elements. This protein has been demonstrated to regulate alternative splicing of tissue specific exons and differentially spliced exons during erythropoiesis.

Especificidad

Other homologies: Bovine (98% sequence homology), Canine (98% sequence homology), Equine (98% sequence homology).

Inmunógeno

MBP-tagged recombinant protein corresponding to mouse Fox2.

Aplicación

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Fox2 in human cerebellum tissue.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected Fox2 in A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Fox2 Antibody (Rabbit Polyclonal Antibody) validated in IHC(P), ICC, WB to detect Fox2 also known as RNA binding protein fox-1 homolog 2, Fox-1 homolog B.

Calidad

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 50 ng/mL of this antibody detected Fox2 in 10 µg of C2C12 cell lysate.

Descripción de destino

~48 kDa observed. Alternative splicing can result in up to 9 isoforms at various molecular weights. Uncharacterized bands between ~60-80 kDa have been observed in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
C2C12 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable