ABE1397

Anti-trimethyl STAT3 (Lys180) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Signal transducer and activator of transcription 3, Acute-phase response factor, trimethyl STAT3 (Lys180)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human (based on 100% sequence homology), porcine (based on 100% sequence homology), orangutan (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• Nº de acceso NCBI: NP_644805
• Nº de acceso UniProt: P40763
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: trimethylation (Lys180 )
• Información sobre el gen: human ... STAT3(6774)

Descripción

Descripción general

Signal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 is known to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). STAT3 gene knockout in mice is lethal at the early embryonic stage.

Especificidad

Trimethylation site is present in all three human spliced isoforms. Reactivity toward Stat3 with mono- or dimethylated Lys180 has not been tested.

Inmunógeno

Epitope: STAT3 K180me3

KLH-conjugated linear peptide corresponding to the STAT3 K180me3 of human trimethyl STAT3 (Lys180)

Aplicación

Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated STAT3 trimethylated on Lys180 in 0.5 mg of mouse embryonic stem cell lysate.
Peptide Inhibition Assay: Target specificity of a representative lot was confirmed by immunogen peptide blocking prior to Western blotting analysis (0.5 µg/mL) ) of mouse embryonic stem cell lysate (10 µg/lane) . Only the immunogen peptide, but not the corresponding peptide without the Lys180 trimethylation modification, blocked the detection of the target band.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-trimethyl STAT3 (Lys180) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay, Immunoprecipitation for the detection of trimethyl STAT3 (Lys180).

Calidad

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAT3 trimethylated on Lys180 in 10 µg of mouse embryonic stem cell lysate.

Descripción de destino

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable