ABC513

Anti-Rab11-FIP1/RCP Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, rat, mouse
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_001002814
• Nº de acceso UniProt: Q6WKZ4
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RAB11FIP1(80223)

Descripción

Descripción general

The protein Rab11-FIP1/RCP, or alternatively named Rab11 family-interacting protein 1, or Rab-coupling protein, and encoded by the human gene RAB11FIP1/RCP is an effector protein that interacts with Rab11 in the processes surrounding endosomal recycling. Rab11-FIP1/RCP also is involved in membrane trafficking in phagocytosis, as well as during axon growth, mediating integrin trafficking in various cancers. RAB11-FIP1/RCP has isoforms. Isoform 1 is non-membrane bound and is localized in the endosome and is involved in endosome recycling. Isoform 2 is more cytoplasmic being confined, for example, to the cytoplasmic phagosome vesicles and the phagosome membrane. Rab11-FIP1/RCP is expressed in multiple tissues and comprises one member of a large family of Rab11 family interacting proteins (Fab11-FIPs) that define discrete subdomains and carry out temporally distinct roles within the recycling and membrane trafficking system of the cell.

Inmunógeno

GST-tagged recombinant protein corresponding to human Rab11-FIP1/RCP.

Aplicación

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Rab11-FIP1/RCP Antibody is validated for use in western blotting, ICC & IP for the detection of Rab11-FIP1/RCP.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab11-FIP1/RCP in 10 µg of C2C12, HEK293, HeLa, HepG2, HUVEC, Jurkat, L6, NIH/3T3, PC-12, PC-3, RAW264.7 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected Rab11-FIP1/RCP in Tac-TGN38 expressing HeLa cell lysates (Jing, J., et al. (2010). Mol Cell Biol. 21(17):3041-3053.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Rab11-FIP1/RCP in Tac-TGN38 expressing HeLa cells (Jing, J., et al. (2010). Mol Cell Biol. 21(17):3041-3053.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Rab11-FIP1/RCP from HeLa cell lysate (Jing, J., et al. (2010). Mol Cell Biol. 21(17):3041-3053.).

Calidad

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab11-FIP1/RCP in 10 µg of A431 cell lysate.

Descripción de destino

~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable